Cardiomyocyte Differentiation Kit-SomaxBio

Cardiomyocyte Differentiation Kit

Catalog No：

R3-01-01

Specification：

1 Kit

Product Introduction：

Developed by Somax Biotechnology, the iPSC-derived Cardiomyocyte Differentiation Kit includes chemically defined, serum-free base medium and additives. It is designed for the directed differentiation of human pluripotent stem cells (hESC/iPSC) into cardiomyocytes. The differentiated cardiomyocytes can be identified by cardiomyocyte-specific proteins—cardiac troponin T (cTnT) and MLC2V key markers, and are suitable for basic and clinical scientific research applications.
The kit consists of Cardiomyocyte Differentiation Medium, Cardiomyocyte Purification Medium, Cardiomyocyte Maintenance Medium, Cardiomyocyte Maturation Medium, and Cardiomyocyte Seeding Medium. Cardiomyocyte Differentiation Medium is used to differentiate iPSCs into cardiomyocytes. If the obtained cardiomyocytes have fewer beating areas, Cardiomyocyte Purification Medium can be used for purification. Cardiomyocyte Seeding Medium is suitable for cardiomyocyte passage. After passage, Cardiomyocyte Maturation Medium can promote the metabolic shift of immature cardiomyocytes from glycolysis to fatty acid oxidation within two weeks, endowing them with better characteristics and application potential. Alternatively, Cardiomyocyte Maintenance Medium can be used after passage to maintain continuous beating of cardiomyocytes for approximately 45 days.

Product Content

Catalog No.	Name	Specification
R3-01-01	Cardiomyocyte Differentiation Medium	250 mL
	Cardiomyocyte Purification Medium	100 mL /500 mL
	Cardiomyocyte Maintenance Medium	100 mL /500 mL
	Cardiomyocyte Maturation Medium	100 mL /500 mL
	Cardiomyocyte Seeding Medium	50 mL

Product Advantages

Stable: Validated by differentiation of iPSC cells from multiple sources, ensuring stable differentiation into cardiomyocytes.

Comprehensive: Equipped with media applicable to various scenarios to meet diverse needs.

Efficient: High yield of cardiomyocytes.

User-friendly: Detailed differentiation procedures; cardiomyocytes can be obtained by simply following the steps.

Differentiation Flow Chart

Operation Procedures and Precautions

Storage and Thawing：Store all components of the kit at the specified temperature for long-term use. Thaw components stored at -20℃ slowly at 4℃ before use, avoid repeated freeze-thaw cycles. It is recommended to aliquot and store according to single-use volume.

Pre-use Inspection：Centrifuge briefly and mix gently before use. Do not use if precipitation, turbidity, or bottle damage is found.

Strictly Follow Protocol：Be sure to use the latest version of the operation manual provided with the kit. Do not arbitrarily adjust steps, time, or reagent ratios to ensure the reproducibility of differentiation results.

Cell State Requirements：Only applicable to iPSC lines with good state and stable pluripotency. The density and state of initial cells are crucial for differentiation efficiency.

Specialized and Pure：All components of the kit are designed specifically for the supporting workflow. Do not mix with other systems. Use sterile techniques during operation to avoid microbial contamination.

Usage Statement：This product is for research use only, not for clinical diagnosis, treatment, or in vivo application in animals/humans.

Experimental Design Suggestion：To ensure experimental success, it is recommended to operate in batches and set cell density gradients when using for the first time.

Product Data

心肌细胞形态

MLC2V

CTNT

iPSC Cell Differentiation Kits
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Frequently Asked Questions

When thawing and seeding iPSC cells, most of them are found to be in a single-cell morphology and hardly attach after 24 hours. What is the cause, and how should it be handled? Precautions for iPSC cell passaging Cells exhibit vacuolization. The significance of establishing iPSC lines from the blood of patients with rare diseases