Ready-to-use CEPT-SomaxBio

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iPSC Culture Reagents

Ready-to-use CEPT

Specification：

150μL

Storage Conditions：

Store each additive component at -80°C for long-term storage. Once opened, store at 2–8°C and use within one month.

Product Advantages

Adding this during cell passage, cryopreservation, and certain differentiation processes can significantly improve experimental results

Significantly reduces cellular stress, improves cell viability, and promotes colony growth

The inhibition of apoptosis and promotion of cell adhesion were more pronounced

Furthermore, prolonged exposure (more than 24 hours) to the culture medium does not increase their tendency toward spontaneous differentiation.

Product Information

Component Code	Name	Specifications	Quantity	Storage Temperature
R2-04-021	CET（2000x）	50 μL	1/10	-80℃
R2-04-022	P（1000x）	100 μL	1/10	-80℃

CEPT Results Showcase

Seed iPSCs at a density of 100,000 cells per well (six-well plate) onto a layer of Soma Stem Cell Seeding Solution (R2-02-01) in iPSC Maintenance Medium (Cat. No.: R2-01-01). Add either CEPT or Y27632 (10 μM) to the culture medium, then remove the solution after 48 hours. It can be observed that CEPT significantly enhances iPSC adhesion compared to Y27632 (D1); iPSCs in CEPT-containing medium exhibit significantly greater expansion capacity than those in Y27632-containing medium (D2); after CEPT removal, the cells rapidly revert to the typical pluripotent stem cell morphology (D3).

iPSC Culture Reagents
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Questions

When thawing and seeding iPSC cells, most of them are found to be in a single-cell morphology and hardly attach after 24 hours. What is the cause, and how should it be handled? Precautions for iPSC cell passaging Cells exhibit vacuolization. The significance of establishing iPSC lines from the blood of patients with rare diseases

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