Neuronal Cell Coating Solution-SomaxBio

Neuronal Cell Coating Solution

Catalog No.：

R2-02-03

Specification：

100 mL

Storage Conditions：

-20℃

Product Introduction：

Although conventional Matrigel coating solutions (such as EHS mouse tumor-derived Matrigel) can support short-term neuronal adhesion and synaptogenesis, due to the degradation of their protein components (such as laminin, type IV collagen) and insufficient mechanical strength, they often struggle to maintain the extension stability of neuronal synapses after long-term culture (exceeding 1 week), leading to synapse loss, axon retraction and other issues. In contrast, Somax's Neuronal Cell Coating Solution can significantly improve this limitation. This coating solution enhances the interaction between neurons and the matrix, maintains long-term extension of neuronal synapses, continuously promotes synaptic plasticity, and ensures structural stability for up to 2 months. It is suitable for research requiring long-term observation of synaptic dynamics, such as Alzheimer's disease models.

Product Advantages

Somax's Neuronal Cell Coating Solution enhances the interaction between neurons and the matrix, maintains long-term extension of neuronal synapses, continuously promotes synaptic plasticity, and ensures structural stability for up to 2 months. It is suitable for research requiring long-term observation of synaptic dynamics, such as Alzheimer's disease models.

Operation Procedures and Precautions

1. Thaw Neuronal Cell Coating Solution 1/2 in a 2~8℃ refrigerator two days in advance.

2. According to the following table, completely cover the bottom of the dish with Neuronal Cell Coating Solution 1 and incubate overnight in a 37℃ incubator. Aspirate and discard after 24 hours, wash twice with PBS, and air-dry with the lid open in a biosafety cabinet. After air-drying, add an equal volume of Neuronal Cell Coating Solution 2 to the same well and incubate in a 37℃ incubator for more than 2 hours, or overnight.

3. When using, take out the coated plate, aspirate and discard Neuronal Cell Coating Solution 2, and add the cell suspension.

Plate Type	Single Well Bottom Area	Volume
6-well plate	9.6 cm²	1 mL
12-well plate	4.5 cm²	500 µL
24-well plate	2 cm²	300 µL
48-well plate	1 cm²	150 µL

Application Scope

This product is for research use only and not for clinical treatment or other purposes.

Cell Characteristics

Product Data

Product Information

Product	Catalog No.	Component Catalog No.	Component Name	Specification
Neuronal Cell Coating Solution	R2-02-03	R2-02-031	Neuronal Cell Coating Solution 1	50 mL
Neuronal Cell Coating Solution	R2-02-03	R2-02-032	Neuronal Cell Coating Solution 2	50 mL

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Questions

When thawing and seeding iPSC cells, most of them are found to be in a single-cell morphology and hardly attach after 24 hours. What is the cause, and how should it be handled? Precautions for iPSC cell passaging Cells exhibit vacuolization. The significance of establishing iPSC lines from the blood of patients with rare diseases