Cardiac Organoid Differentiation Kit-SomaxBio

Cardiac Organoid Differentiation Kit

Catalog No：

R3-01-02

Specification：

1 Kit

Storage Conditions：

Base Medium: 4℃; Additives: -20℃

Product Introduction：

Developed by Somax Biotechnology, the Cardiac Organoid Differentiation Kit includes chemically defined, serum-free base medium and additives. This ready-to-use organoid kit is used for the self-organization of human induced pluripotent stem cells (iPSCs) into miniature cardiac-like structures under three-dimensional culture conditions, mimicking the developmental process, tissue structure, and functional characteristics of the human heart. It has broad application prospects in disease modeling, drug screening, and regenerative medicine, and is suitable for basic and clinical scientific research.

Product Content

Catalog No.	Name	Specification
R3-01-02	EB Induction Medium	50 mL
	Cardiac Organoid Differentiation Medium A	20 mL
	Cardiac Organoid Differentiation Medium B	20 mL
	Cardiac Organoid Differentiation Medium C	40 mL
	Cardiac Organoid Differentiation Medium D	100 mL
	Cardiac Organoid Maintenance Medium	50 mL

Product Advantages

Convenient: This product is a chemically defined, serum-free ready-to-use kit.

Superior to Traditional 2D Cardiomyocyte Culture: Cardiac organoids are more similar to real cardiac tissue in terms of cell type diversity, spatial structure complexity, and physiological function performance.

Long-term Maintenance: Can be maintained in maintenance medium for more than 40 days.

Differentiation Flow Chart

Operation Procedures and Precautions

Storage and Thawing：Store all components of the kit at the specified temperature for long-term use. Thaw components stored at -20℃ slowly at 4℃ before use, avoid repeated freeze-thaw cycles. It is recommended to aliquot and store according to single-use volume.

Pre-use Inspection：Centrifuge briefly and mix gently before use. Do not use if precipitation, turbidity, or bottle damage is found.

Strictly Follow Protocol：Be sure to use the latest version of the operation manual provided with the kit. Do not arbitrarily adjust steps, time, or reagent ratios to ensure the reproducibility of differentiation results.

Cell State Requirements：Only applicable to iPSC lines with good state and stable pluripotency. The density and state of initial cells are crucial for differentiation efficiency.

Specialized and Pure：All components of the kit are designed specifically for the supporting workflow. Do not mix with other systems. Use sterile techniques during operation to avoid microbial contamination.

Usage Statement：This product is for research use only, not for clinical diagnosis, treatment, or in vivo application in animals/humans.

Experimental Design Suggestion：To ensure experimental success, it is recommended to operate in batches and set cell density gradients when using for the first time.

Product Data

CTNT

Merge

WT1

Product Data

Morphology of Cardiac Organoid Differentiation

Cardiac Organoids Express cTNT and WT1

iPSC Organoid Differentiation Kits
Other Products

Cardiac Organoid Differentiation Kit Brain Organoid Kit Renal Organoid Differentiation Kit

Frequently Asked Questions

When thawing and seeding iPSC cells, most of them are found to be in a single-cell morphology and hardly attach after 24 hours. What is the cause, and how should it be handled? Precautions for iPSC cell passaging Cells exhibit vacuolization. The significance of establishing iPSC lines from the blood of patients with rare diseases