iMSC Differentiation Kit-SomaxBio

iMSC Differentiation Kit

Catalog No：

R3-04-01

Specification：

1 Kit

Storage Conditions：

Base Medium: 4℃; Additives: -20℃

Product Introduction：

Developed by Somax Biotechnology, the iMSC Differentiation Kit efficiently converts human induced pluripotent stem cells (iPSCs) into mesenchymal stem cells (MSCs). The obtained mesenchymal-like cells (iPSC-MSCs) are highly similar to primary MSCs in terms of gene expression profile, surface marker characteristics, and multi-directional differentiation potential. They continuously and stably express MSC markers such as CD90, CD73, and CD105, do not express non-specific markers such as CD45, CD34, and HLA-DR, and maintain the tri-lineage differentiation potential of osteogenesis, adipogenesis, and chondrogenesis. This kit is suitable for basic and clinical scientific research.

Product Content

Catalog No.	Name	Specification
R3-04-01	iMSC Differentiation Medium A	50 mL
	iMSC Differentiation Medium B	200 mL
	iMSC Maintenance Medium	200 mL/500 mL

Product Advantages

Advanced Differentiation Technology: Adopts advanced small-molecule directed differentiation technology to efficiently convert iPSCs into therapeutic potential mesenchymal stem cells (iMSCs).

Similar to Primary MSCs: Through precise regulation of the differentiation process, the obtained mesenchymal-like cells (iMSCs) are highly similar to primary MSCs in terms of gene expression profile, surface marker characteristics, and multi-directional differentiation potential.

Stable Marker Expression: Continuously and stably expresses mesenchymal stem cell markers such as CD90, CD73, and CD105; does not express non-specific markers such as CD45, CD34, and HLA.

Tri-lineage Differentiation Potential: Maintains the tri-lineage differentiation potential of osteogenesis, adipogenesis, and chondrogenesis.

Differentiation Flow Chart

分化流程图.jpg

Operation Procedures and Precautions

Storage and Thawing：Store all components of the kit at the specified temperature for long-term use. Thaw components stored at -20℃ slowly at 4℃ before use, avoid repeated freeze-thaw cycles. It is recommended to aliquot and store according to single-use volume.

Pre-use Inspection：Centrifuge briefly and mix gently before use. Do not use if precipitation, turbidity, or bottle damage is found.

Strictly Follow Protocol：Be sure to use the latest version of the operation manual provided with the kit. Do not arbitrarily adjust steps, time, or reagent ratios to ensure the reproducibility of differentiation results.

Cell State Requirements：Only applicable to iPSC lines with good state and stable pluripotency. The density and state of initial cells are crucial for differentiation efficiency.

Specialized and Pure：All components of the kit are designed specifically for the supporting workflow. Do not mix with other systems. Use sterile techniques during operation to avoid microbial contamination.

Usage Statement：This product is for research use only, not for clinical diagnosis, treatment, or in vivo application in animals/humans.

Experimental Design Suggestion：To ensure experimental success, it is recommended to operate in batches and set cell density gradients when using for the first time.

Product Data