Service Features：

Featured Gene Editing Services: Specialized lncRNA knockout services and iPSC-specific gene editing services are available.

No Exogenous Gene Integration: Delivered via RNP electroporation, avoiding random insertion mutations caused by lentiviral vectors and off-target effects caused by CRISPR/Cas9 integration.

High Efficiency and Short Turnaround Time: Gene knockout projects can be completed in as little as 2 months, with 100% correctly modified monoclonal cell lines delivered.

Professional Services: One-to-one communication with dedicated technical support, a detailed feasibility analysis report prior to project kickoff, and full professional technical support throughout the project cycle.

Follow-up Supporting Technical Services: RNA-seq services are available to support mechanistic research such as the identification of relevant signaling pathways.

Case Introduction:

Case│iPSC CYFIP2 c.259C>T Point Mutation | Partner: Hunan Provincial Maternal and Child Health Care Hospital

Result Verification: The sequencing results of the mixed cells are shown below (sequenced with reverse primer, the base selected in blue is the c.259C>T target mutation site). It can be seen from the figure that after electroporation of sgCYFIP2, a red peak T appears at the c.259C>T position, indicating that a certain amount of "c.259C>T" was mutated back to "T" in the mixed cells. Subsequently, single clone picking and identification were performed, and the mutant cell line was obtained in a single round of screening.