Fluorescently Labeled iPSCs (GFP/mCherry)-SomaxBio

Fluorescently Labeled iPSCs (GFP/mCherry)

Catalog No.：

See Product Information

Specification：

1×10⁶cells/vial

Storage Conditions：

Liquid nitrogen

Product Introduction：

This product is a human induced pluripotent stem cell (iPSC-GFP/mCherry) reporter cell line independently developed by Somax. It is efficiently induced from peripheral blood mononuclear cells (PBMCs) via non-integrating reprogramming technology. High-efficiency transduction is achieved by constructing plasmid vectors and using a lentiviral packaging system, enabling stable integration and continuous expression of green/red fluorescent protein genes in the target cell genome, thereby obtaining long-term traceable fluorescent labeling effects.

Green Fluorescent Label:

pLVX-EGFP-Puro

Red Fluorescent Label:

pGWLV24-mCherry-Puro

Product Advantages

Maintains stable and rapid proliferation ability with normal karyotype during long-term culture

Exhibits typical clonal morphology and pluripotency gene expression characteristics highly similar to hESCs

Confirmed to have complete three-germ-layer differentiation potential in in vitro directed differentiation

Stable expression of GFP/mCherry labeling provides a visualization tool for cell tracing research. This cell line has undergone strict quality control and is an ideal standardized tool for stem cell basic research, drug screening, and regenerative medicine application

Operation Procedures and Precautions

After receiving the cells, observe and record any signs of thawing during transportation and the integrity of the cryovials.

Transfer to liquid nitrogen immediately; temporary storage at -80℃ is allowed, but prolonged storage is strictly prohibited.

Recommended recovery culture system for cryovials: 2 wells of a 6-well plate.

Application Scope：

This product is for research use only and not for clinical treatment or other purposes.

Cell Characteristics

iPSC-GFP Pre-cryopreservation

iPSC-GFP Post-recovery

iPSC-mCherry Pre-cryopreservation

iPSC-mCherry Post-recovery

Product Information

Product Name	Catalog No.	Source
Green Fluorescent Labeled iPSCs (GFP)	JY04-01-01	Healthy Donor-Derived iPSCs
Red Fluorescent Labeled iPSCs (mCherry)	JY04-02-01	Healthy Donor-Derived iPSCs

iPSC Cell Products
Other Products

Healthy Donor-Derived iPSCs Disease Model iPSCs Fluorescently Labeled iPSCs (GFP/mCherry) NGN2-Stable iPSCs

Questions

When thawing and seeding iPSC cells, most of them are found to be in a single-cell morphology and hardly attach after 24 hours. What is the cause, and how should it be handled? Precautions for iPSC cell passaging Cells exhibit vacuolization. The significance of establishing iPSC lines from the blood of patients with rare diseases