NGN2-Stable iPSCs-SomaxBio

NGN2-Stable iPSCs

Catalog No.：

JY05-01-01

Specification：

1×10⁶cells/vial

Storage Conditions：

Liquid nitrogen

Product Introduction：

This product is generated by introducing the neurogenic transcription factor Neurogenin-2 (NGN2) gene into healthy donor-derived iPSCs via lentiviral vector. The exogenous NGN2 gene is controlled by the tetON promoter and rtTA transregulatory element, enabling stable expression upon Doxcyclin induction. When used in conjunction with Somax's NGN2-mediated Neuron Differentiation Kit (Catalog No.: R3-02-02), this cell line can efficiently and rapidly differentiate into mature functional neurons.

Product Advantages

Neurons differentiated from this product exhibit high reproducibility and temporal controllability, enabling the acquisition of a high-purity (>90%) neuron population in a short period (usually 2 weeks)

Good batch-to-batch stability, suitable for large-scale drug screening

Operation Procedures and Precautions

After receiving the cells, observe and record any signs of thawing during transportation and the integrity of the cryovials.

Transfer to liquid nitrogen immediately; temporary storage at -80℃ is allowed, but prolonged storage is strictly prohibited.

Recommended recovery culture system for cryovials: 2 wells of a 6-well plate.

Application Scope：

This product is for research use only and not for clinical treatment or other purposes.

Cell Characteristics

Cell state bright field (10X)

Fluorescent dTomato (10X)

Product Information

Cell Type	Catalog No.	Specification
NGN2-Stable iPSCs	JY05-01-01	1×10⁶ cells/vial

iPSC Cell Products
Other Products

Healthy Donor-Derived iPSCs Disease Model iPSCs Fluorescently Labeled iPSCs (GFP/mCherry) NGN2-Stable iPSCs

Questions

When thawing and seeding iPSC cells, most of them are found to be in a single-cell morphology and hardly attach after 24 hours. What is the cause, and how should it be handled? Precautions for iPSC cell passaging Cells exhibit vacuolization. The significance of establishing iPSC lines from the blood of patients with rare diseases