Forebrain Neuron Differentiation Kit-SomaxBio

Forebrain Neuron Differentiation Kit

Catalog No：

R3-02-01

Specification：

1 Kit

Storage Conditions：

Base Medium: 4℃; Additives: -20℃

Product Introduction：

The Forebrain Neuron Differentiation Kit is a high-efficiency cell differentiation tool independently developed by Somax Biotechnology, specifically designed for the directed differentiation of human pluripotent stem cells (hESC/iPSC) or neural progenitor cells into functionally mature forebrain glutamatergic neurons. Through optimized combinations of small-molecule compounds and growth factor formulations, this product ensures an efficient and stable differentiation process (differentiation efficiency ≥ 90%). The obtained neurons can specifically express FOXG1 (a key transcription factor for forebrain development), MAP2 (microtubule-associated protein), and VGLUT1 (a marker vesicular transport protein for glutamatergic neurons). It is suitable for basic and clinical scientific research applications.

Product Content

Catalog No.	Name	Specification
R3-02-01	Neuron Differentiation Medium A	20 mL
	Neuron Differentiation Medium B	50 mL
	Neuron Differentiation Medium C	50 mL
	Neuron Differentiation Medium D	100 mL
	Neuron Maintenance Medium	200 mL

Product Advantages

Suitable for Neuroscience Research: Applicable to studies on intellectual disability, neurodegenerative diseases, neuromuscular diseases, etc.

Optimized Formulation: Optimized combinations of small-molecule compounds and growth factors ensure efficient and stable differentiation (differentiation efficiency ≥ 90%).

Specific Marker Expression: Specifically expresses FOXG1 (key forebrain development transcription factor), MAP2 (microtubule-associated protein), and VGLUT1 (glutamatergic neuron marker vesicular transport protein).

Differentiation Flow Chart

前脑神经元分化流程图.png

Operation Procedures and Precautions

Storage and Thawing：Store all components of the kit at the specified temperature for long-term use. Thaw components stored at -20℃ slowly at 4℃ before use, avoid repeated freeze-thaw cycles. It is recommended to aliquot and store according to single-use volume.

Pre-use Inspection：Centrifuge briefly and mix gently before use. Do not use if precipitation, turbidity, or bottle damage is found.

Strictly Follow Protocol：Be sure to use the latest version of the operation manual provided with the kit. Do not arbitrarily adjust steps, time, or reagent ratios to ensure the reproducibility of differentiation results.

Cell State Requirements：Only applicable to iPSC lines with good state and stable pluripotency. The density and state of initial cells are crucial for differentiation efficiency.

Specialized and Pure：All components of the kit are designed specifically for the supporting workflow. Do not mix with other systems. Use sterile techniques during operation to avoid microbial contamination.

Usage Statement：This product is for research use only, not for clinical diagnosis, treatment, or in vivo application in animals/humans.

Experimental Design Suggestion：To ensure experimental success, it is recommended to operate in batches and set cell density gradients when using for the first time.

Product Data

FOXG1-DAPI

MAP2-DAPI

VGLUT1-DAPI

iPSC Cell Differentiation Kits
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Frequently Asked Questions

When thawing and seeding iPSC cells, most of them are found to be in a single-cell morphology and hardly attach after 24 hours. What is the cause, and how should it be handled? Precautions for iPSC cell passaging Cells exhibit vacuolization. The significance of establishing iPSC lines from the blood of patients with rare diseases