This product is ready-to-use and can be aliquoted for storage after opening. Taking one well of a 6-well plate as an example: aspirate and discard the supernatant, add 1 mL PBS for washing, aspirate and discard the PBS, add 1 mL EDTA Digestive Solution to each well, incubate in a 37℃ incubator for 2~3 minutes, and end the incubation when loose cracks appear in the cell colonies under the microscope. Transfer to a biosafety cabinet, aspirate and discard the EDTA, aspirate 1-2 mL DMEM/F12, pipette the cells up and down twice each to break the colonies into small clumps of 10-50 cells. Collect the cells into a centrifuge tube and centrifuge at 300g for 3 minutes.