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Tri-lineage Differentiation Characterization Kit A
Catalog No:
R4-01-02A
Specification:
1Kit,5 tests
Storage Conditions:
Store at -20°C. Valid for 1 year.
Product Introduction:
The Tri-lineage Differentiation Characterization Kit A, independently developed by SomaxBio, is an efficient and reliable assay tool specially designed for accurate detection following the differentiation of iPSCs/ESCs into three germ layers. This kit targets FOXA2, Brachyury and PAX6, specific markers for endoderm, mesoderm and ectoderm respectively. All primary antibodies are formulated at a unified dilution ratio for immunofluorescence staining. Combined with the secondary antibodies supplied in the kit, it enables specific characterization of three-germ-layer differentiation. Validated in multiple rounds of experiments, it delivers clear and consistent results.
Product Content

Kit Contents:

Component Catalog No.

Name

Specification

Quantity

Storage Temperature

R4-01-02A1

Rabbit-Anti-PAX6

5.5 μL

1 vial

-20℃

R4-01-02A2

Rabbit-Anti-Brachyury

5.5 μL

1 vial

-20℃

R4-01-02A3

Rabbit-Anti-FOXA2

5.5 μL

1 vial

-20℃

R4-01-02A4

Donkey Anti-Rabbit-594

7.5 μL

1 vial

2-8℃

* All primary antibodies contain 40% glycerol and 0.05% sodium azide. They will not freeze at -20°C and aliquoting is not required.

Staining Result Gallery

Endoderm

Mesoderm

Ectoderm

Scale bar in all images: 50 μm.

Operation Procedures and Precautions

This product is supplied in small-volume single-tube aliquots. Briefly centrifuge (200 RCF, 30 s) before first use upon receipt to ensure all reagents are collected at the bottom of the tube.

Intended Use Statement: This product is for research use only. It is not intended for clinical diagnosis, treatment, or in vivo application in humans or animals.